MicroRNA-17-5p acts as a biomarker and regulates mitochondrial dynamics in trophoblasts and endothelial cells by targeting the mitofusins Mfn1/Mfn2 in gestational diabetes mellitus

Introduction Gestational diabetes mellitus (GDM) is a metabolic disease that endangers pregnant women and their offspring. Insights into biomarkers GDM pathogenesis are crucial. Ectopic expression of microRNA-17-5p was found in GDM, but its the diagnostic value role miR-17-5p remain unclear. Material methods Detection miRNA microarray quantitative PCR (qPCR) significantly increased positively associated with biochemical indicators 30 plasma samples 28 matched control samples. Results The area under ROC curve 0.827 (P < 0.01), which showed good potential. Mitochondrial staining compared controls, trophoblasts exhibited more mitochondrial fusion endothelial cells fission than these controls. Western blot qPCR assays further revealed mitofusin Mfn1/Mfn2 lower primary from patients, whereas higher patients those Conversely, Bioinformatics luciferase reporter confirmed both Mfn1 Mfn2 targets miR-17-5p. Last, decreased Mfn1/2 observed not only to increase apoptotic rate also reverse anti-apoptotic effects inhibitor. Conclusions MiR-17-5p regulates Mfn1/Mfn2-mediated dynamics involved GDM. may serve as promising biomarker therapeutic target for